Reviewing the Fabrication of Nanopores Using TEM
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Loading... - Alice Ankomah
- 09 Sep 2022
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Despite the commercialization of biological nanopore-based sequencing methods, the solid-state nanopore is preferable due to its mechanical and chemical stability, size tunability, and ease of integration into measurement electronics.
However, a quick, cost-effective, and easy solid-state nanopore fabrication method with large-scale production is unavailable, limiting its practical applicability. Transmission electron microscope (TEM) based fabrication is a frequently used nanopore fabrication technique in laboratory-level research due to its ability to image and fabricate nanopores in parallel.
The review article published in the journal Micron focused on various aspects of nanopore technology based on TEM. Hybrid nanopores, prepared by DNA origami integration into solid-state nanopores, were highlighted as hybrid particles leveraging the benefits of biological and solid-state nanopores.
Nanopore Technology
Nanopore technology involves nano-scale holes embedded in a thin membrane structure to detect the potential change when charged biological molecules smaller than nanopore pass through the hole. Therefore, nanopore technology has the potential to sense and analyze single-molecule amino acids, DNA, RNA, and many more.
DNA sequencing methods based on nanopores are new techniques, providing a low-cost and portable method without the requirement for DNA amplification or restrictions on the length of the DNA to be sequenced.
The present article focused on nanopore-based devices with nanometer-scale pores fabricated on a thin membrane that separates the two chambers filled with electrolytes. Voltage application between the two chambers permits the passage of molecules and ions between the chambers. Read More...
